Select any girl in gallery mode. Leave the cursor alone once the selection is made and do not touch your controller. After a minute or so, the selected girl will start talking.
Becky - Clear the story mode using Candy Cane
Doctor Cutter - Clear the story mode using Anesthesia
Judgment - Clear the story mode using Bloody Shadow
Killer Khan - Clear the story mode using Aigle
Mistress Spencer - Clear the story mode using Miss Spencer
Noble Rose - Clear the story mode using Evil Rose
Rowdy Reiko - Clear the story mode using Reiko Hinomoto
Sergeant Clemets - Clear the story mode using Dixie Clemets
Showbiz - Clear the story mode using Aisha
The BBD - Clear the story mode using Makoto Aihara
Gallery Mode - Win the title with any girl
Beach Mode - Defend your title with any girl
Beat story mode with every character to unlock Lady X. Beat it with their alter ego to unlock Lady X Substance.